mhqN [2020-02-20 12:11:45]

similar to NAD(P)H nitroreductase

locus: BSU_05480

pI: 5.56

mw: 23.41 kDa

protein length: 206 aa

gene length: 621 bp

function: unknown

product: unknown

essential: no

synonyms: ydfN

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 596,478 597,098

The protein

Protein family

• [SW|nitroreductase family] (according to UniProt)

Structure

• [PDB|3BEM]

Expression and Regulation

Operons

• genes: [gene|A0842DD5F1B13AC15C95F18751E7836DF31B262A|mhqN]-[gene|ACAFB2072E74CC059B492A31EF492028B96C569F|mhqO]-[gene|B4384C8C0874BAA7822E9B6DCAB57790018271D8|mhqP]
  description: [Pubmed|17725564]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|17725564], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|997B828A99D6D8460712C18ED0CE566B285C22DC|MhqR]: repression, [Pubmed|17725564], in [regulon|997B828A99D6D8460712C18ED0CE566B285C22DC|MhqR regulon]

  regulation

  • induced by catechol and 2-methylhydroquinone ([protein|search|MhqR]) [Pubmed|17725564]

Biological materials

Mutant

• MGNA-C153 (ydfN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2151 NBRP B. subtilis, Japan]
• BKE05480 ([gene|A0842DD5F1B13AC15C95F18751E7836DF31B262A|mhqN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACGCTACACTCCCTTT, downstream forward: _UP4_TATATGTAAAAGGAGCGGAT
• BKK05480 ([gene|A0842DD5F1B13AC15C95F18751E7836DF31B262A|mhqN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACGCTACACTCCCTTT, downstream forward: _UP4_TATATGTAAAAGGAGCGGAT

References

• 17407181,17725564,27965289